This is done by sterilizing the grain in jacketed mixers which heat and cool in fast cycles and grain introduction into the already sterilized and cooled by proprietary methods and then mixed. The mixed, and spawned substrate is filled into the PE bags and sealed to ... amount of oxygen is needed, the plastic will be stripped off anyway. The incubation room is generally maintained at a constant 65-67°F. When fruiting is needed, a well water soaking will induce fruiting. mycology sterilizer part 65 This way your lab is sterilized and remains sterilized as only clean air is pushed in and any time the door is open the airflow is out of the lab and into the rest of the house. Thus reducing the new contaminates entering the lab. 
I wanted to post an update to my experiences about using SpawnMate SEII and ProMycel as a fruiting substrate supplementation added prior to sterilization of a fruiting substrate to be inoculated. ... To give you an example, 120ml of a vigorous Pan Cyan liquid culture will colonize a large gallon-sized spawn bag about 50% to 70% in four days if the spawn bag contains my usual 65% moisture content manure/WBS blend (10% WBS per dry weight). If I use SpawnMate,& ... 
It`s basically an outline of how to deeply integrate mycology and edible mushroom propagation into a small permaculture farm system, point by point. Thank you ... Acclimated to northern latitudes, this mushroom fruits when air temperatures range between 60-90° F (15-32° C) which usually translates to ground temperatures of 55-65° F (13-18° C). ... Indoors, these mushrooms can be grown on sterilized substrates or on logs using the methods described in this book. 
This is done by sterilizing the grain in jacketed mixers which heat and cool in fast cycles and grain introduction into the already sterilized and cooled by proprietary methods and then mixed. The mixed, and spawned substrate is filled into the PE bags and sealed to ... amount of oxygen is needed, the plastic will be stripped off anyway. The incubation room is generally maintained at a constant 65-67°F. When fruiting is needed, a well water soaking will induce fruiting. 
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